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Table 3 Effect of amino acid location (buried, exposed) on organic solvent resistance of BSLA when replacing each type of amino acid in BSLA WT

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

  Location of amino acid positions in BSLA (%)
Exposed positions (128; 71%) Buried positions (53; 29%)
  + (275) = (1777) − (319) × (189) + (96) = (435) − (220) × (309)
DMSO
 Aromatic 10 15 22 15 14 12 16 19
 Aliphatic 25 27 21 17 21 36 20 14
 Polar 31 36 33 38 44 36 41 26
 Charged 35 23 24 30 22 15 23 41
  + (138) = (1903) − (330) × (189) + (21) = (385) − (345) × (309)
DOx
 Aromatic 16 15 17 15 10 12 15 19
 Aliphatic 17 27 19 17 14 39 20 14
 Polar 26 35 35 38 52 40 37 26
 Charged 41 23 28 30 24 9 28 41
  + (152) = (1883) − (336) × (189) + (29) = (394) − (328) × (309)
TFE
 Aromatic 19 15 15 15 17 12 15 19
 Aliphatic 16 27 24 17 10 37 22 14
 Polar 30 36 32 38 38 40 38 26
 Charged 34 23 29 30 34 11 26 41
  1. Sixteen patterns per organic solvent are shown. The absolute number of BSLA variants per type is shown in brackets
  2. Amino acid groups: aromatic (F, Y, and W), aliphatic (A, V, L, I, and G), charged (D, E, H, K, and R), and polar (C, M, P, S, T, N, and Q). For activity group definitions (+, =, −, ×) see text. Values are given as percentages for easier comparison, as BSLA amino acid composition is not evenly distributed (43.6% aliphatic, 29.8% polar, 18.2% charged, 8.3% aromatic). The number of inactive variants (×) was the same for all organic solvents. Column “×” shows that the number of inactive variants resulted from amino acid substitutions. The activity was measured in a buffer plate which was used as reference (absence of organic solvent) for comparison. Therefore, the number of inactive variants was the same for all four organic solvents