| |
Location of amino acid positions in BSLA (%)
|
|---|
|
Exposed positions (128; 71%)
|
Buried positions (53; 29%)
|
|---|
| |
+ (275)
|
= (1777)
|
− (319)
|
× (189)
|
+ (96)
|
= (435)
|
− (220)
|
× (309)
|
|
DMSO
|
|
Aromatic
|
10
|
15
|
22
|
15
|
14
|
12
|
16
|
19
|
|
Aliphatic
|
25
|
27
|
21
|
17
|
21
|
36
|
20
|
14
|
|
Polar
|
31
|
36
|
33
|
38
|
44
|
36
|
41
|
26
|
|
Charged
|
35
|
23
|
24
|
30
|
22
|
15
|
23
|
41
|
| |
+ (138)
|
= (1903)
|
− (330)
|
× (189)
|
+ (21)
|
= (385)
|
− (345)
|
× (309)
|
|
DOx
|
|
Aromatic
|
16
|
15
|
17
|
15
|
10
|
12
|
15
|
19
|
|
Aliphatic
|
17
|
27
|
19
|
17
|
14
|
39
|
20
|
14
|
|
Polar
|
26
|
35
|
35
|
38
|
52
|
40
|
37
|
26
|
|
Charged
|
41
|
23
|
28
|
30
|
24
|
9
|
28
|
41
|
| |
+ (152)
|
= (1883)
|
− (336)
|
× (189)
|
+ (29)
|
= (394)
|
− (328)
|
× (309)
|
|
TFE
|
|
Aromatic
|
19
|
15
|
15
|
15
|
17
|
12
|
15
|
19
|
|
Aliphatic
|
16
|
27
|
24
|
17
|
10
|
37
|
22
|
14
|
|
Polar
|
30
|
36
|
32
|
38
|
38
|
40
|
38
|
26
|
|
Charged
|
34
|
23
|
29
|
30
|
34
|
11
|
26
|
41
|
- Sixteen patterns per organic solvent are shown. The absolute number of BSLA variants per type is shown in brackets
- Amino acid groups: aromatic (F, Y, and W), aliphatic (A, V, L, I, and G), charged (D, E, H, K, and R), and polar (C, M, P, S, T, N, and Q). For activity group definitions (+, =, −, ×) see text. Values are given as percentages for easier comparison, as BSLA amino acid composition is not evenly distributed (43.6% aliphatic, 29.8% polar, 18.2% charged, 8.3% aromatic). The number of inactive variants (×) was the same for all organic solvents. Column “×” shows that the number of inactive variants resulted from amino acid substitutions. The activity was measured in a buffer plate which was used as reference (absence of organic solvent) for comparison. Therefore, the number of inactive variants was the same for all four organic solvents
