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Table 3 Effect of amino acid location (buried, exposed) on organic solvent resistance of BSLA when replacing each type of amino acid in BSLA WT

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

 

Location of amino acid positions in BSLA (%)

Exposed positions (128; 71%)

Buried positions (53; 29%)

 

+ (275)

= (1777)

− (319)

× (189)

+ (96)

= (435)

− (220)

× (309)

DMSO

 Aromatic

10

15

22

15

14

12

16

19

 Aliphatic

25

27

21

17

21

36

20

14

 Polar

31

36

33

38

44

36

41

26

 Charged

35

23

24

30

22

15

23

41

 

+ (138)

= (1903)

− (330)

× (189)

+ (21)

= (385)

− (345)

× (309)

DOx

 Aromatic

16

15

17

15

10

12

15

19

 Aliphatic

17

27

19

17

14

39

20

14

 Polar

26

35

35

38

52

40

37

26

 Charged

41

23

28

30

24

9

28

41

 

+ (152)

= (1883)

− (336)

× (189)

+ (29)

= (394)

− (328)

× (309)

TFE

 Aromatic

19

15

15

15

17

12

15

19

 Aliphatic

16

27

24

17

10

37

22

14

 Polar

30

36

32

38

38

40

38

26

 Charged

34

23

29

30

34

11

26

41

  1. Sixteen patterns per organic solvent are shown. The absolute number of BSLA variants per type is shown in brackets
  2. Amino acid groups: aromatic (F, Y, and W), aliphatic (A, V, L, I, and G), charged (D, E, H, K, and R), and polar (C, M, P, S, T, N, and Q). For activity group definitions (+, =, −, ×) see text. Values are given as percentages for easier comparison, as BSLA amino acid composition is not evenly distributed (43.6% aliphatic, 29.8% polar, 18.2% charged, 8.3% aromatic). The number of inactive variants (×) was the same for all organic solvents. Column “×” shows that the number of inactive variants resulted from amino acid substitutions. The activity was measured in a buffer plate which was used as reference (absence of organic solvent) for comparison. Therefore, the number of inactive variants was the same for all four organic solvents