Fig. 2
From: Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol
Construction of recombinant vector for dhaD expression. PlacUV5 modified Plac promotor for expression of the eco47IR gene at a level sufficient to allow for positive selection, eco47IR lethal gene enables positive selection of recombinant plasmid, dhaD inserted gene glycerol dehydrogenase, PT7 T7 RNA polymerase promotor for in vitro transcription of the cloned insert, AMP gene resistant to ampicillin, rep replicon from pMBI plasmid responsible for the replication of pJET1.2