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Table 2 Substrate spectrum of OYE2p

From: Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal

Substrate Structure No. Specific activity (U/mg) ee (%)/config.
Maleimide 1a 6.0
N-Phenylmaleimide 2a 4.24
2-Cyclopentenone 3a 2.49
Ketoisophorone 4a 3.05 25(R)
(R)-Carvone 5a 0.54 74(2R,5R)
(S)-Carvone 6a 0.7 98(2R,5S)
(S)-(−)-Perilla aldehyde 7a 2.71 nd
(S)-(−)-Perillyl alcohol 8a 0.18 nd
2-Pentenal 9a 2.04
Trans-2-hexenal 10a 1.58
2-Octenal 11a 1.1
2-Methyl-2-pentenal 12a 1.61 nd
2-Isopropyl-5-methyl-2-hexenal 13a 0.98 nd
Neral 14a 0.28 26.5(R)
Geranial 15a 0.31 98.9(R)
α-Methylcinnamaldehyde 16a 2.96 20(R)
Cinnamaldehyde 17a 2.53
Cinnamylalcohol 18a 0.2
Fumaric acid 19a 1.1
Dimethyl maleate 20a 3.8
Fumaramide 21a 0.88
Fumaric dinitrile 22a 1.76
2-Butenenitrile 23a 1.07
  1. Specific activities were determined at 30 °C using 5 mM substrate and 0.2 mM NADH in 200 mM PBS (pH 7.4)
  2. nd not determined