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Table 2 Substrate spectrum of OYE2p

From: Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal

Substrate

Structure

No.

Specific activity (U/mg)

ee (%)/config.

Maleimide

1a

6.0

N-Phenylmaleimide

2a

4.24

2-Cyclopentenone

3a

2.49

Ketoisophorone

4a

3.05

25(R)

(R)-Carvone

5a

0.54

74(2R,5R)

(S)-Carvone

6a

0.7

98(2R,5S)

(S)-(−)-Perilla aldehyde

7a

2.71

nd

(S)-(−)-Perillyl alcohol

8a

0.18

nd

2-Pentenal

9a

2.04

Trans-2-hexenal

10a

1.58

2-Octenal

11a

1.1

2-Methyl-2-pentenal

12a

1.61

nd

2-Isopropyl-5-methyl-2-hexenal

13a

0.98

nd

Neral

14a

0.28

26.5(R)

Geranial

15a

0.31

98.9(R)

α-Methylcinnamaldehyde

16a

2.96

20(R)

Cinnamaldehyde

17a

2.53

Cinnamylalcohol

18a

0.2

Fumaric acid

19a

1.1

Dimethyl maleate

20a

3.8

Fumaramide

21a

0.88

Fumaric dinitrile

22a

1.76

2-Butenenitrile

23a

1.07

  1. Specific activities were determined at 30 °C using 5 mM substrate and 0.2 mM NADH in 200 mM PBS (pH 7.4)
  2. nd not determined