Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Bioresources and Bioprocessing

Table 2 Tolerance improved strains obtained by forward genetics approaches

Parental strain	Technique(s)	Mutant(s)	Improved phenotype	References
ZM4	ALE	ZMF3-3	Cell growth, glucose utilization, and ethanol yield under 3 g/L furfural	Shui et al. (2015)
ZM4	ALE	ZMA7-2	Cell growth and glucose utilization under 7 g/L acetic acid	Shui et al. (2015)
8b	ALE	#7	Sugars utilization and ethanol production in corn stover liquor	Mohagheghi et al. (2014)
8b	ALE	SS3	Xylose utilization and ethanol yields using corn stover hydrolysate	Mohagheghi et al. (2015)
ZM4	NTG mutagenesis	ZM482	Molasses utilization and ethanol productivity	Rogers et al. (1984)
ZM4	NTG mutagenesis	AcR	Ethanol productivity and rate under 20 g/L sodium acetate	Joachimsthal et al. (1998)
ZM4	NTG mutagenesis	ZM401	Cell growth, glucose utilization, and ethanol production under 10.5 g/L acetic acid or 1.0 g/L vanillin	Lee et al. (1982); Zhao et al. (2014)
ZM4	Transposon mutagenesis	ZMT2	Sugar conversion rate to ethanol under 2% NaCl stress	Wang et al. (2016)
ZM4	NTG mutagenesis and ALE	ZMNTG6014	Performance under acetic acid (1.4 or 1.6%)	Wang (2008)
ZM481	NTG mutagenesis and ALE	ZMA-142, ZMA-167	Glucose utilization and ethanol production under 244 mM sodium acetate	Liu et al. (2017)
33C	Transposon mutagenesis and ALE	OEL19, 22, 23, 26, 31	Cell growth under 2-4 g/L furfural	Yang et al. (2014b)
ZM4	gTME (error-prone PCR)	MF1, 2, 3	Glucose utilization under 3 g/L furfural	Tan et al. (2015)