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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

Fig. 3

Phylogenetic and functional analyses of TapA in P. pastoris. a Phylogenetic clustering of putative statin efflux pumps (TapA) with major facilitator superfamily (MFS) transporters involved in the efflux of toxic compounds. Branch TapA is boldfaced, and the GenBank accession numbers and protein names of the sequences are shown. The length of the scale bar is equivalent to 0.1 substitutions of amino acids per site. b Subcellular localization of TapA in P. pastoris. c Growth comparison of GS_P GAP -tapA and wild-type P. pastoris GS115 on YPD plates containing gradient concentrations of lovastatin. d Growth comparison between GS_P GAP -tapA and wild-type P. pastoris GS115 in the liquid YPD medium containing lovastatin in shaking flasks. Three replicates were tested and experiments were conducted three times

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