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Table 3 Effect of metal irons and chemicals on purified recombinant enzyme Egls activities

From: Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Metal ions Relative activity (%) Chemicals Relative activity (%)
2 MM 10 MM 2% (w/v) 5% (w/v)
Control 100.3 ± 1.0 100.3 ± 1.0 Control 100.3 ± 1.0 100.3 ± 1.0
Ca2+ 105.2 ± 2.3 115.7 ± 5.2a Triton-X 100 105.0 ± 4.4 122.9 ± 5.8a
K+ 105.5 ± 1.8 104.3 ± 4.6 Twen-20 100.8 ± 3.6 105.2 ± 2.5
Mg2+ 111.0 ± 1.4a 134.8 ± 11.1a EDTA 99.6 ± 0.6 96.7 ± 0.5
Mn2+ 101.7 ± 1.7 134.2 ± 4.9a PMSF ND ND
Ni2+ 98.8 ± 3.1 98.6 ± 3.6    
Zn2+ 100.3 ± 3.4 122.0 ± 1.8a    
Cu2+ 100.0 ± 3.0 103.5 ± 4.8    
Co2+ 110.1 ± 1.1a 116.5 ± 3.8a    
  1. ND not detected
  2. aSignificant difference (p < 0.05)