Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Bioresources and Bioprocessing

Table 3 Effect of metal irons and chemicals on purified recombinant enzyme Egls activities

Metal ions	Relative activity (%)		Chemicals	Relative activity (%)
Metal ions	2 MM	10 MM	Chemicals	2% (w/v)	5% (w/v)
Control	100.3 ± 1.0	100.3 ± 1.0	Control	100.3 ± 1.0	100.3 ± 1.0
Ca²⁺	105.2 ± 2.3	115.7 ± 5.2^a	Triton-X 100	105.0 ± 4.4	122.9 ± 5.8^a
K⁺	105.5 ± 1.8	104.3 ± 4.6	Twen-20	100.8 ± 3.6	105.2 ± 2.5
Mg²⁺	111.0 ± 1.4^a	134.8 ± 11.1^a	EDTA	99.6 ± 0.6	96.7 ± 0.5
Mn²⁺	101.7 ± 1.7	134.2 ± 4.9^a	PMSF	ND	ND
Ni²⁺	98.8 ± 3.1	98.6 ± 3.6
Zn²⁺	100.3 ± 3.4	122.0 ± 1.8^a
Cu²⁺	100.0 ± 3.0	103.5 ± 4.8
Co²⁺	110.1 ± 1.1^a	116.5 ± 3.8^a