Fig. 3From: Azoreductase: a key player of xenobiotic metabolismProposed azoreductase mechanism for Amaranth azo dye degradation: step 1. Upon oxidation of NADH, the hydride is transferred to FMN present in the active site of azoreductase. Substrate (Amaranth azo dye) binds to one of the active sites of azoreductase. Then the reduced FMN transfers the hydride to Amaranth azo dye. Step 2 is another cycle of hydride transfer from NADH to Amaranth azo dye via FMNBack to article page