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Table 5 Expression of genes involved in transporter mechanisms

From: Transcriptomic analysis of Aspergillus niger strains reveals the mechanism underlying high citric acid productivity

Gene ID (1015) Gene ID (513.88) Predicted function (homolog and organism)a FPKM-10 h FPKM-40 h Fold changeb
S1 S3 S2 S1 S3 S2 S1/S3 (10 h) S1/S2 (10 h) S1/S3 (40 h) S1/S2 (40 h)
2.412.1 An02g08970 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily) 185.2 2.21 94.74 21.26 4.17 5.31 6.38 2.44 2.00
3000074 An08g10970 Synaptic vesicle transporter SVOP and related transporters 160.28 0.71 917.73 6.31 9.60 11.21 7.80 − 2.51
160123 An17g01710 Synaptic vesicle transporter SVOP and related transporters 103.76 2.77 49.29 930.44 2.51 122.66 5.22 1.07 8.53 2.92
5000334 An07g05880 Synaptic vesicle transporter SVOP and related transporters 107.56 4.57 49.04 70.54 8.35 23.06 4.55 1.13 3.07 1.61
5.1708.1 No Synaptic vesicle transporter SVOP and related transporters 24.18 2.10 58.59 323.21 43.80 63.16 3.52 − 1.27 2.88 2.35
160129 An10g00690 Permease of the major facilitator superfamily 118.95 0.44 26.12 13.04 6.55 0.91 8.07 2.18 3.83
9000464 An12g03550 Permease of the major facilitator superfamily 80.14 1.01 28.94 53.88 13.55 1.50 6.29 1.46 1.99 5.16
31842 An08g03200 Ammonia permease 658.74 11.45 452.98 874.63 583.09 67.70 5.84 3.69
8.781.1 An14g02390 Ammonia permease 95.62 5.25 9.21 6.99 7.80 5.15 4.18 3.37
150043 No Amino acid transporters 313.46 3.98 494.76 1872.15 1170.98 219.82 6.30 3.09
18.284.1 An16g07900 Amino acid transporters 91.62 2.61 2.59 60.14 8.98 12.63 5.13 5.14 2.74 2.25
11.172.1 An09g02550 Amino acid transporters 85.21 1.01 3.44 178.80 4.02 13.43 6.39 4.63 5.47 3.73
19000022 An03g01590 Amino acid transporters 77.90 0.49 6.71 79.07 7.25 1.22 7.32 3.53 3.44 6.01
51440 An07g01950 Xanthine/uracil transporters (A. nidulans, uapC) 103.74 21.22 60.84 245.16 194.55 16.35 2.28 3.90
31302 An08g06240 Uridine permease/thiamine transporter/allantoin transport 60.46 4.80 28.19 269.89 72.22 3.70 3.65 1.10 1.90 6.18
110148 No Tetrapeptide transporter, OPT1/isp4 1213.89 6.69 1523.02 1328.97 230.62 666.57 6.18 2.52
18.109.1 An16g06770 Monocarboxylate transporter 45.49 6.18 11.67 106.08 12.00 24.68 2.87 1.95 3.14 2.10
111102 An09g00660 Oligopeptide transporter OPT superfamily 20.89 0.33 96.18 570.88 25.23 123.98 6.00 − 2.20 4.49 2.20
21.45.1 An12g10320 Fe2+/Zn2+ regulated transporter 111.77 5.31 126.14 2585.52 606.42 499.30 4.39 2.09 2.37
10845 An01g03790 Na+: solute symporter 74.05 3.14 53.95 51.89 51.48 2.18 4.56 4.57
1001002 An01g01950 Mg2+ transporter protein, CorA-like 42.43 0.98 12.76 461.93 187.17 408.91 5.44 1.73 1.30
9000143 An12g07730 Ctr Cu2+ transporter 79.63 16.88 94.62 518.01 27.41 58.48 2.23 4.24 3.14
22.68.1 An04g09850 git1, Inorganic phosphate transporter 16.87 1.25 20.28 100.07 22.88 14.74 3.75 2.12 2.76
240077 An19g00340 Ca2+/H+ antiporter VCX1 and related proteins 50.22 0.86 15.81 615.05 81.96 175.32 5.77 1.58 2.98 1.89
240035 An19g00330 Ca2+/H+ antiporter VCX1 and related proteins 29.77 2.50 15.81 204.99 28.69 92.59 3.57 2.83 1.15
  1. “–” denotes genes with no differences at transcriptional level, fold change ≤ 1. Some of the genes have small differences, but have a decisive impact on CA production. We also used fold change as an indicator
  2. “No” denotes that sequence was not mapped to the genome sequence assembly of A. niger strain CBS 513.88
  3. a Description of gene or closest homolog (BLASTP)
  4. b Fold change of differentially expressed (FDR < 0.05, FPKM ≥ 50 in A. niger YX-1217 at time 10 or time 40) genes based on a comparison of transcription levels at YX-1217/ATCC 1015 (designated as S1/S3) (10 h), YX-1217/YX-1217G (designated as S1/S2) (10 h), YX-1217/ATCC 1015 (designated as S1/S3) (40 h), and YX-1217/YX-1217G (designated as S1/S2) (40 h), respectively