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Table 7 Gene expression verification by qRT-PCR

From: Transcriptomic analysis of Aspergillus niger strains reveals the mechanism underlying high citric acid productivity

Gene ID Predicted function (homolog and organism) RNA-Seq qRT-PCR
S1/S3 (10 h) S1/S2 (10 h) S1/S3 (40 h) S1/S2 (40 h) S1/S3 (10 h) S1/S2 (10 h) S1/S3 (40 h) S1/S2 (40 h)
190032 GH 10, endo-1,4-beta-xylanase F1 11.24 9.96 6.05 2.58 10.69 8.42 5.50 1.93
80523 eglA, GH 12, endoglucanase A 7.9 5.43 4.3 − 2.12 6.3 4.21 4.1 − 1.1
190140 axhA, GH 62, alpha-l-arabinofuranosidase 8.36 7.59 8.47 3.08 7.41 6.38 6.54 2.01
6000119 mscA, methylcitrate synthase 3.79 2.58 0.31 0.20 3.45 2.45 0.20 0.10
12707 Peptidase aspartic, active site (AP1) (A. phoenicis, apnS) 2.80 1.75 5.23 1.11 2.11 1.54 4.37 1.00
7000050 Acyl-CoA synthetase 4.47 1.06 3.86 0.45 3.98 1.01 3.55 0.22
160123 Synaptic vesicle transporter SVOP and related transporters 5.22 1.07 8.53 2.92 6.45 0.67 9.36 2.44
11.172.1 Amino acid transporters 6.39 4.63 5.47 3.73 7.65 5.00 6.57 2.84
  1. All data were ratio log2
  2. Genes based on a comparison of transcription levels at YX-1217/ATCC 1015 (designated as S1/S3) (10 h), YX-1217/YX-1217G (designated as S1/S2) (10 h), YX-1217/ATCC 1015 (designated as S1/S3) (40 h), and YX-1217/YX-1217G (designated as S1/S2) (40 h), respectively