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Table 7 Gene expression verification by qRT-PCR

From: Transcriptomic analysis of Aspergillus niger strains reveals the mechanism underlying high citric acid productivity

Gene ID

Predicted function (homolog and organism)

RNA-Seq

qRT-PCR

S1/S3 (10 h)

S1/S2 (10 h)

S1/S3 (40 h)

S1/S2 (40 h)

S1/S3 (10 h)

S1/S2 (10 h)

S1/S3 (40 h)

S1/S2 (40 h)

190032

GH 10, endo-1,4-beta-xylanase F1

11.24

9.96

6.05

2.58

10.69

8.42

5.50

1.93

80523

eglA, GH 12, endoglucanase A

7.9

5.43

4.3

− 2.12

6.3

4.21

4.1

− 1.1

190140

axhA, GH 62, alpha-l-arabinofuranosidase

8.36

7.59

8.47

3.08

7.41

6.38

6.54

2.01

6000119

mscA, methylcitrate synthase

3.79

2.58

0.31

0.20

3.45

2.45

0.20

0.10

12707

Peptidase aspartic, active site (AP1) (A. phoenicis, apnS)

2.80

1.75

5.23

1.11

2.11

1.54

4.37

1.00

7000050

Acyl-CoA synthetase

4.47

1.06

3.86

0.45

3.98

1.01

3.55

0.22

160123

Synaptic vesicle transporter SVOP and related transporters

5.22

1.07

8.53

2.92

6.45

0.67

9.36

2.44

11.172.1

Amino acid transporters

6.39

4.63

5.47

3.73

7.65

5.00

6.57

2.84

  1. All data were ratio log2
  2. Genes based on a comparison of transcription levels at YX-1217/ATCC 1015 (designated as S1/S3) (10 h), YX-1217/YX-1217G (designated as S1/S2) (10 h), YX-1217/ATCC 1015 (designated as S1/S3) (40 h), and YX-1217/YX-1217G (designated as S1/S2) (40 h), respectively