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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: 2-Hydroxy-4-(3′-oxo-3′H-benzofuran-2′-yliden)but-2-enoic acid biosynthesis from dibenzofuran using lateral dioxygenation in a Pseudomonas putida strain B6-2 (DSM 28064)

Fig. 3

Trials of biosynthesis conditions. DBF degradation (dotted line) and HOBB accumulation (solid line) were evaluated under different conditions. a Initial DBF concentration. DBF degradation: 0.3-mM DBF (open square), 0.5-mM DBF (open circle), 0.8-mM DBF (open triangle), 1.0-mM DBF (open diamond); HOBB accumulation: 0.3-mM DBF (closed square), 0.5-mM DBF (closed circle), 0.8-mM DBF (closed triangle), and 1.0-mM DBF (closed diamond). b Different pH values. DBF degradation: pH 5 (open square), pH 7 (open circle), pH 8 (open triangle), pH 9 (open diamond); HOBB accumulation: pH 5 (closed square), pH 7 (closed circle), pH 8 (closed triangle), pH 9 (closed diamond). c Cells density. DBF degradation: OD600 2 (open square), OD600 5 (open circle), OD600 10 (open triangle); HOBB accumulation: OD600 2 (closed square), OD600 5 (closed circle), and OD600 10 (closed triangle). d DBF degradation (open square) and HOBB accumulation (closed square) under the best conditions among all trials: OD600 5, pH 7, and CDBF = 0.5 mM in a 10-mL system

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