TY - JOUR AU - Bankefa, Olufemi Emmanuel AU - Wang, Meiyu AU - Zhu, Taicheng AU - Li, Yin PY - 2018 DA - 2018/05/26 TI - Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris JO - Bioresources and Bioprocessing SP - 25 VL - 5 IS - 1 AB - A mixed feeding strategy (co-feeding of complex carbon sources with methanol) has become a common practice for process development in Pichia pastoris to increase cell biomass and enzyme production levels. However, in some cases mixed feeding did not have a significant impact or even had a negative effect on specific enzyme productivity. We hypothesized that this may be due to a bottleneck in the protein secretion pathway caused by too strong protein expression as a result of mixed feeding operation. SN - 2197-4365 UR - https://doi.org/10.1186/s40643-018-0211-y DO - 10.1186/s40643-018-0211-y ID - Bankefa2018 ER -