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Table 1 Strains and plasmids used in the study

From: Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris

Plasmids/strains Descriptions Reference
Plasmids
 pPICZ Vector for extracellular expression recombinant vector carrying Invitrogen
 pPICZαA Vector for extracellular expression Invitrogen
 pPICHα Recombinant vector carrying hygromycin gene from Klebsiella pneumonia This study
 pPICZα-HsXbp1 pPICZαA-based vector carrying Hac1p from H. sapiens; Zeor This study
 pPICZα-PpHac1p pPICZαA-based vector carrying Hac1p from P. pastoris; Zeor This study
 pPICHα-Gox pPICHα-based vector carrying glucose oxidase gene This study
Strains
 E. coli DH5α Commercial transformation host for cloning Takara
 P. pastoris GS115 Commercial transformation host for
Cloning; his4, Mut+
Invitrogen
 GSgox GS115 integrated with inducible glucose oxidase gene This study
 GSgox-Hs GS115-Gox integrated with linearized pPICZαA-HsHac1p This study
 GSgox-Pp GS115-Gox integrated with linearized pPICZαA-PpHac1p This study