Table 1 Strains and plasmids used in the study
Plasmids/strains | Descriptions | Reference |
|---|---|---|
Plasmids | ||
pPICZ | Vector for extracellular expression recombinant vector carrying | Invitrogen |
pPICZαA | Vector for extracellular expression | Invitrogen |
pPICHα | Recombinant vector carrying hygromycin gene from Klebsiella pneumonia | This study |
pPICZα-HsXbp1 | pPICZαA-based vector carrying Hac1p from H. sapiens; Zeor | This study |
pPICZα-PpHac1p | pPICZαA-based vector carrying Hac1p from P. pastoris; Zeor | This study |
pPICHα-Gox | pPICHα-based vector carrying glucose oxidase gene | This study |
Strains | ||
E. coli DH5α | Commercial transformation host for cloning | Takara |
P. pastoris GS115 | Commercial transformation host for Cloning; his4−, Mut+ | Invitrogen |
GSgox | GS115 integrated with inducible glucose oxidase gene | This study |
GSgox-Hs | GS115-Gox integrated with linearized pPICZαA-HsHac1p | This study |
GSgox-Pp | GS115-Gox integrated with linearized pPICZαA-PpHac1p | This study |