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Table 4 The knockout targets which have been validated in our lab and on other published papers

From: In silico reconstruction and experimental validation of Saccharopolyspora erythraea genome-scale metabolic model iZZ1342 that accounts for 1685 ORFs

Gene ORF Reaction Gene function Genetic engineered in related references
mutB SACE_5639 Succinyl-CoA[c] <=> (R) methylmalonyl-CoA[c] Methylmalonyl-CoA mutase (MCM) Chen et al. (2016); Weber et al. (2012)
eryG SACE_0728 ErythD[c] + amet[c] => erythb[c] + ahcys[c]
ErythC[c] + amet[c] => erythromycin[c] + ahcys[c]
Erythromycin C methyltransferase Mironov et al. (2004)
eryK SACE_0731 6(S)-Methylmalonyl-CoA[c] + propanoyl-CoA[c] + 6NADPH[c] + 6 H[c] <=> 7CoA[c] + 6-deoxyerythronolide-B[c] + 6CO2[c] + 6NADP[c] + H2O[c] eryBIII NDP-4-keto-2,6-dideoxyhexose 3-C-methyltransferase Minas et al. (1998)
sucC SACE_6669 ADP[c] + orthophosphate[c] + succinyl-CoA[c] <=> ATP[c] + succinate[c] + CoA[c] Succinyl-CoA synthetase subunit β Hong et al. (2017)