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Table 4 The knockout targets which have been validated in our lab and on other published papers

From: In silico reconstruction and experimental validation of Saccharopolyspora erythraea genome-scale metabolic model iZZ1342 that accounts for 1685 ORFs

Gene

ORF

Reaction

Gene function

Genetic engineered in related references

mutB

SACE_5639

Succinyl-CoA[c] <=> (R) methylmalonyl-CoA[c]

Methylmalonyl-CoA mutase (MCM)

Chen et al. (2016); Weber et al. (2012)

eryG

SACE_0728

ErythD[c] + amet[c] => erythb[c] + ahcys[c]

ErythC[c] + amet[c] => erythromycin[c] + ahcys[c]

Erythromycin C methyltransferase

Mironov et al. (2004)

eryK

SACE_0731

6(S)-Methylmalonyl-CoA[c] + propanoyl-CoA[c] + 6NADPH[c] + 6 H[c] <=> 7CoA[c] + 6-deoxyerythronolide-B[c] + 6CO2[c] + 6NADP[c] + H2O[c]

eryBIII NDP-4-keto-2,6-dideoxyhexose 3-C-methyltransferase

Minas et al. (1998)

sucC

SACE_6669

ADP[c] + orthophosphate[c] + succinyl-CoA[c] <=> ATP[c] + succinate[c] + CoA[c]

Succinyl-CoA synthetase subunit β

Hong et al. (2017)