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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Gene mining-based identification of aldo–keto reductases for highly stereoselective reduction of bulky ketones

Fig. 3

Substrate specificities of the enzymes towards the tested carbonyl substrates, including (1a) methyl benzoylformate, (2a) ethyl benzoylformate, (3a) ethyl 2-oxo-4-phenylbutyrate, (4a) ethyl benzoylacetate, and (5a) 1-benzyl-3-pyrrolidinone. The reaction mixture consisted of 0.1 M sodium phosphate buffer (pH 6.5), 0.5 mM NADPH, 5 mM substrate, and the appropriate enzyme in a total volume of 100 µL. The experiments were carried out in triplicate, and the error bars represent the standard error of the mean

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