Fig. 4

Apparent kinetic parameters of the enzymes, including a CaCR, b ScCR, c KmCR, d CPR-C1, and e CPR-C2 in reduction of the carbonyl substrates. The primary Y-axis “K_m(mM)/k_cat(s⁻¹)” means “K_m(mM) or k_cat(s⁻¹)”. Enzymatic activities were assayed at various concentrations of each substrate (0.05–4 mM) with NADPH at saturated concentrations. Tetrahydrofuran (1% v/v) was used as the cosolvent for dissolution of carbonyl substrates