Table 2 Transformation of S. oneidensis MR-1 for over-expression of GFP with different ori and promoter
From: Turn on the Mtr pathway genes under pLacI promoter in Shewanella oneidensis MR-1
Plasmids | Ori | Size (bp) | Resistance | CFU result | GFP function | Culture picture |
|---|---|---|---|---|---|---|
pETSXM2-pLacI-GFP | f1, pBR322, repB-SXM | 4626 | Kan (50) | 888 | Yes |
|
pETSXM2-proA-GFP | f1, pBR322, repB-SXM | 4290 | Kan (50) | 358 | No |
|
pETSXM2-proB-GFP | f1, pBR322, repB-SXM | 4395 | Kan (50) | 288 | No |
|
pSB1C3-repB-GFP | pMB1, repB-SXM | 4219 | Cm (25) | 1000 | Yes |
|
pSB1C3-GFP | pMB1 | 3153 | Cm (25) | 4 | Yes |
|
