TY - JOUR AU - Li, Zhen-Hai AU - Wang, Feng-Qing AU - Wei, Dong-Zhi PY - 2018 DA - 2018/07/30 TI - Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae JO - Bioresources and Bioprocessing SP - 36 VL - 5 IS - 1 AB - Saccharomyces cerevisiae is one of the most important industrial microorganisms. A robust genome editing tool is vital for both fundamental research and applications. To save the time and labor consumed in the procedure of genome editing, a self-cloning CRISPR/Cpf1 system (scCRISPR/Cpf1), in which a self-cleaving plasmid and PCR-generated site-specific crRNA fragment were included, was developed. SN - 2197-4365 UR - https://doi.org/10.1186/s40643-018-0222-8 DO - 10.1186/s40643-018-0222-8 ID - Li2018 ER -