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Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae

Fig. 1

Schematic of scCRISPR/Cpf1. a pSC-Pal: the self-cleaving complementary palindromic crRNA and Cpf1 co-expression plasmid. b Schematic shows the scCRISPR/Cpf1 process that occurs inside target yeast cells. CEN6/ARS4, replication origin; URA3, selection marker used in yeast; AmpR, selection marker used in E. coli; PTEF1-Cpf1, the Cpf1 expression cassette driven by promoter PTEF1; DR, 19 bp direct repeat which shown in black; PAM, protospacer-associated motif sequence; PSNR52, SNR52 promoter; TSUP4, SUP4 terminator; the complementary palindromic sequence of crRNA was shown in brown and the site-specific sequence of crRNA was shown in red

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