Fig. 5
From: Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae
Application of scCRISPR/Cpf1 in metabolic pathway engineering. a Construction and optimization of the synthesis pathway of patchoulol in S. cerevisiae. Black single arrows represent one-step conversions. Black double arrows represent multiple steps. Green arrows represent the overexpressed genes. Blue arrow represents the downregulated gene by PHXT1 promoter. Red cross represents the deleted gene. HMG-CoA, 3-hydroxy-3-methylglutaryl coenzyme A; IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate; IDI1, isopentenyl pyrophosphate isomerase; FPP, farnesyl diphosphate; tHMG1, truncated 3-hydroxy-3-methylglutaryl-coenzyme-A reductase; ERG9, squalene synthase; LPP1 and DPP1, both encoding lipid phosphate phosphatases; FPPs: farnesyl diphosphate synthase; and FPPs-PTs, fusion gene of farnesyl diphosphate synthase and patchoulol synthase. b (Left) The patchoulol titer produced at various time points in strains BY4741, BY-PT-001, and BY-PT-003, respectively. (Right) The growth curves of strains BY4741, BY-PT-001, and BY-PT-003, respectively