Table 1 The main plasmids and strains used in this study
From: Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae
Plasmids or strains | Characteristics | Source or reference |
|---|---|---|
Plasmid | ||
 p416XWP01 | URA3, CEN6/ARS4 ori, AmpR | Xie et al. (2013) |
 pMRI-35-crtYB-crtI | PTEF1-crtI-TTPS1, PHXT7-crtYB-TPGK1 | Xie et al. (2013) |
 pMRI-34-crtE-tHMG1 | TCYC1-tHMG1-PGal1-PGal10-crtE-TADH1 | Xie et al. (2013) |
 pUMRI-15 | PTEF1-TTPS1, KanR | Xie et al. (2013) |
 p426-PSNR52-gRNA | PSNR52, AmpR | Addgene (43,803) |
 pUMRI-15-Cpf1 | PTEF1-Cpf1-TTPS1, KanR | This study |
 p416XWP01-Cpf1 | p416XWP01, PTEF1-Cpf1-TTPS1, AmpR | This study |
 pSC-Pal | p416XWP01, PTEF1-Cpf1-TTPS1, PSNR52-Pal-TSUP4, AmpR | This study |
 pUMRI-15-MEL1 | pUMRI-15, PTEF1-MEL1-TTPS1, KanR | This study |
 pUMRI-15-FPPs-PTS | pUMRI-15, PTEF1-FPPs-PTs-TTPS1, KanR | This study |
 p416XWP01-IDI1 | p416XWP01, PGal10-IDI1-TADH1, AmpR | This study |
Strain | ||
 BY4741 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | Brachmann et al. (1998) |
 BY-PT-001 | BY4741, Gal1-7::PTEF1-FPPs-PTs-TTPS1, Gal80:: PGal1-tHMG1-TCYC1, HO:: PGal10- IDI1- TADH1, crRNA and Cpf1 co-expression plasmid | This study |
 BY-PT-002 | BY4741, Gal1-7::PTEF1-FPPs-PTs-TTPS1, Gal80:: PGal1-tHMG1-TCYC1, HO:: PGal10- IDI1- TADH1, | This study |
 BY-PT-003 | BY-PT-002, ∆LPP1, ∆DPP1, PERG9::PHXT1, crRNA and Cpf1 co-expression plasmid | This study |