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Table 1 The main plasmids and strains used in this study

From: Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae

Plasmids or strains Characteristics Source or reference
Plasmid
 p416XWP01 URA3, CEN6/ARS4 ori, AmpR Xie et al. (2013)
 pMRI-35-crtYB-crtI PTEF1-crtI-TTPS1, PHXT7-crtYB-TPGK1 Xie et al. (2013)
 pMRI-34-crtE-tHMG1 TCYC1-tHMG1-PGal1-PGal10-crtE-TADH1 Xie et al. (2013)
 pUMRI-15 PTEF1-TTPS1, KanR Xie et al. (2013)
 p426-PSNR52-gRNA PSNR52, AmpR Addgene (43,803)
 pUMRI-15-Cpf1 PTEF1-Cpf1-TTPS1, KanR This study
 p416XWP01-Cpf1 p416XWP01, PTEF1-Cpf1-TTPS1, AmpR This study
 pSC-Pal p416XWP01, PTEF1-Cpf1-TTPS1, PSNR52-Pal-TSUP4, AmpR This study
 pUMRI-15-MEL1 pUMRI-15, PTEF1-MEL1-TTPS1, KanR This study
 pUMRI-15-FPPs-PTS pUMRI-15, PTEF1-FPPs-PTs-TTPS1, KanR This study
 p416XWP01-IDI1 p416XWP01, PGal10-IDI1-TADH1, AmpR This study
Strain
 BY4741 MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 Brachmann et al. (1998)
 BY-PT-001 BY4741, Gal1-7::PTEF1-FPPs-PTs-TTPS1, Gal80:: PGal1-tHMG1-TCYC1, HO:: PGal10- IDI1- TADH1, crRNA and Cpf1 co-expression plasmid This study
 BY-PT-002 BY4741, Gal1-7::PTEF1-FPPs-PTs-TTPS1, Gal80:: PGal1-tHMG1-TCYC1, HO:: PGal10- IDI1- TADH1, This study
 BY-PT-003 BY-PT-002, ∆LPP1, ∆DPP1, PERG9::PHXT1, crRNA and Cpf1 co-expression plasmid This study