Fig. 5
Cellulase production and a comparison of transcript levels of cellulase-related genes between TACE1VP and RUT C30. FPase (A), pNPCase (B), CMCase (C), and pNPGase (D) activities of RUT C30 and TACE1VP after the switch from glycerol to 20 g/L Avicel or lactose. Enzymatic activities were measured at 2, 3, and 4 days after the transfer. Error bars show the respective standard deviations of three biological replicates; asterisks indicate significant differences (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s. not significant) between the transformants and RUT C30, as assessed by Student’s t test. Expression ratios of cellulase-related genes on 20 g/L Avicel (E) or lactose (F) for 12 and 24 h after the switch from glycerol. The data on TXYR1VP transformants were normalized to the corresponding gene expression at the same time points in RUT C30. Values represent the mean of three biological replicates, and the error bars denote standard deviation. Gene expression ratios greater than twofold or less than 0.5-fold are marked with “a”