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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Fig. 3

Two-stage fermentation carried out in a 1.5-L bioreactor by MLB/pTrc99a-pck using different kinds of glycerol as carbon source. a Crude glycerol (B-1); b simply purified crude glycerol (B-2); c pure glycerol (B-3). About 10 g/L of ammonium acetate were added at the beginning of the aerobic stage in these three different fermentations as the co-carbon sources with glycerol

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