Strain, PDB ID, CE class | Enzyme and fold | Catalytic residues and reaction type | CATH and Pfam | Substrates and Refs |
---|---|---|---|---|
Lactobacillus johnsonii, [3PF8], CE-1 | Cinnamoyl esterase α/β hydrolases | Ser106, His225, Asp197 Serine esterase | 3-Layer α/β sandwich Rossmann fold α/β hydrolase family | Ferulic acid, Caffeic acid and ethyl ferulate. Cinnamoyl esterase is active against short acyl chain aliphatic esters and phenolic esters (Lai et al. 2011) |
Aspergillus niger, [1UWC], CE-1 | Feruloyl esterase α/β hydrolases | Ser133, His247, Asp194 Serine esterase | 3-Layer α/β sandwich Rossmann fold Lipase class 3 | Arabinoxylans (hydrolysis of feruloyl-arabinose ester bond) pectin (feruloyl-galactose ester). High substrate specificity towards esters where feruloyl group is attached to C-5 of arabinose and inactive against C-2 esters (McAuley et al. 2004) |
Sporotrichum thermophile, [4G4J], CE-15 | Glucuronoyl esterase, α/β hydrolases | Ser213, Glu236, His346 Serine hydrolase | 3-Layer α/β sandwich Rossmann fold | It has high specificity for hydrolyzing the ester bonds of 4-O-methyl-d-glucuronic acid units of glucuronoxylan and alcohols of lignin (Charavgi et al. 2013) |