Run | Factors | Responses | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Temperature (°C) | Time (h) | Agitation (oscillation/min) | Inoculum (%) | Molasses (%) | Peptone (g/L) | Yeast extract (g/L) | K2HPO4 (g/L) | Surface tension (mN/m) | Inhibition diameters (mm) | Yield (%) | |
1 | 32.5 (0) | 48.0 (0) | 150.0 (0) | 2.0 (0) | 5.5 (0) | 5.5 (0) | 3.5 (0) | 2.0 (0) | 46.4 | 28.0 | 1.40 |
2 | 25.0 (− 1) | 24.0 (− 1) | 100.0 (− 1) | 1.0 (− 1) | 2.0 (− 1) | 3.0 (− 1) | 2.0 (− 1) | 1.0 (− 1) | 59.9 | 12.5 | 0.70 |
3 | 40.0 (+ 1) | 72.0 (+ 1) | 100.0 (− 1) | 3.0 (+ 1) | 2.0 (− 1) | 3.0 (− 1) | 2.0 (− 1) | 3.0 (+ 1) | 50.1 | 16.0 | 0.80 |
4 | 40.0 (+ 1) | 24.0 (− 1) | 200.0 (+ 1) | 1.0 (− 1) | 2.0 (− 1) | 3.0 (− 1) | 5.0 (+ 1) | 3.0 (+ 1) | 60.5 | 13.0 | 0.75 |
5 | 32.5 (0) | 48.0 (0) | 150.0 (0) | 2.0 (0) | 5.5 (0) | 5.5 (0) | 3.5 (0) | 2.0 (0) | 45.1 | 20.0 | 1.00 |
6 | 25.0 (− 1) | 72.0 (+ 1) | 100.0 (− 1) | 1.0 (− 1) | 2.0 (− 1) | 8.0 (+ 1) | 5.0 (+ 1) | 3.0 (+ 1) | 51.0 | 15.0 | 0.85 |
7 | 32.5 (0) | 48.0 (0) | 150.0 (0) | 2.0 (0) | 5.5 (0) | 5.5 (0) | 3.5 (0) | 2.0 (0) | 43.2 | 20.5 | 1.02 |
8 | 25.0 (− 1) | 72.0 (+ 1) | 200.0 (+ 1) | 3.0 (+ 1) | 2.0 (− 1) | 8.0 (+ 1) | 5.0 (+ 1) | 1.0 (− 1) | 49.3 | 15.0 | 0.85 |
9 | 25.0 (− 1) | 24.0 (− 1) | 100.0 (− 1) | 3.0 (+ 1) | 9.0 (+ 1) | 8.0 (+ 1) | 2.0 (− 1) | 3.0 (+ 1) | 43.1 | 19.0 | 0.95 |
10 | 40.0 (+ 1) | 24.0 (− 1) | 100.0 (− 1) | 1.0 (− 1) | 9.0 (+ 1) | 8.0 (+ 1) | 5.0 (+ 1) | 1.0 (− 1) | 39.0 | 49.0 | 2.15 |
11 | 25.0 (− 1) | 24.0 (− 1) | 200.0 (+ 1) | 3.0 (+ 1) | 9.0 (+ 1) | 3.0 (− 1) | 5.0 (+ 1) | 3.0 (+ 1) | 49.6 | 8.4 | 0.42 |
12 | 40.0 (+ 1) | 72.0 (+ 1) | 100.0 (− 1) | 3.0 (+ 1) | 9.0 (+ 1) | 3.0 (− 1) | 5.0 (+ 1) | 1.0 (− 1) | 44.6 | 28.0 | 1.40 |
13 | 40.0 (+ 1) | 24.0 (− 1) | 200.0 (+ 1) | 3.0 (+ 1) | 2.0 (− 1) | 8.0 (+ 1) | 2.0 (− 1) | 1.0 (− 1) | 44.0 | 25.0 | 1.25 |
14 | 25.0 (− 1) | 72.0 (+ 1) | 200.0 (+ 1) | 1.0 (− 1) | 9.0 (+ 1) | 3.0 (− 1) | 2.0 (− 1) | 1.0 (− 1) | 49.0 | 28.0 | 1.40 |
15 | 40.0 (+ 1) | 72.0 (+ 1) | 200.0 (+ 1) | 1.0 (− 1) | 9.0 (+ 1) | 8.0 (+ 1) | 2.0 (− 1) | 3.0 (+ 1) | 37.8 | 52.0 | 2.30 |