Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum

Bioresources and Bioprocessing

Table 5 Cost comparison for various materials in fermentation medium

Category	Component	Cost ($/t)^a	Condition (kg/m³)
Category	Component	Cost ($/t)^a	Medium (Fan et al. 2014)^b	Medium (Du et al. 2017)^c	Medium (this study)
Carbon source	Glucose	400	0	5	0
	Fructose	1000	10	0	0
	Sucrose	600	0	2	0
	Cane molasses	150	0	0	63
	Glycerol	1000	27	40	23
Organic nitrogen source	Yeast extract	3500	10	10	0
Organic nitrogen source	Soybean powder	500	0	0	3
AP-3 titer (mg/L)			144	117	141
Total ($/t)			72	78	34

^aFeedstock cost (US $/t): the unit prices of various components are derived from the Chinese eBay “Alibaba” website
^bThe detail component in medium (Fan et al. 2014) was fructose 9.37 g/L, glycerol 26.78 g/L, yeast extract 10 g/L, buckwheat filtrate 20 g/L, soluble starch 3.03 g/L, MgSO₄·7H₂O 0.5 g/L, FeSO₄·7H₂O 0.01 g/L, KH₂PO₄, 0.25 g/L, NH₄Cl 1 g/L, CaCO₃ 5 g/L, and pH 7.4. Fermentation was carried out in 250 mL flasks containing 50 mL of medium and the cells were cultivated at 28 °C, 220 rpm for 8 days
^cThe detailed component in medium (Du et al. 2017) was glucose 5 g/L, glycerol 40 g/L, sucrose 2.39 g/L, yeast extract 10 g/L, KH₂PO₄·3H₂O 0.65 g/L, MgSO₄·7H₂O 0.5 g/L, FeSO₄·7H₂O 0.002 g/L, CaCO₃ 2 g/L, and pH 7.45. Fermentation was carried out in 250 mL flasks containing 60 mL of medium and the cells were cultivated at 28 °C, 220 rpm for 12 days