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Table 5 Cost comparison for various materials in fermentation medium

From: Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum

Category Component Cost ($/t)a Condition (kg/m3)
Medium (Fan et al. 2014)b Medium (Du et al. 2017)c Medium (this study)
Carbon source Glucose 400 0 5 0
Fructose 1000 10 0 0
Sucrose 600 0 2 0
Cane molasses 150 0 0 63
Glycerol 1000 27 40 23
Organic nitrogen source Yeast extract 3500 10 10 0
Soybean powder 500 0 0 3
AP-3 titer (mg/L)    144 117 141
Total ($/t)    72 78 34
  1. aFeedstock cost (US $/t): the unit prices of various components are derived from the Chinese eBay “Alibaba” website
  2. bThe detail component in medium (Fan et al. 2014) was fructose 9.37 g/L, glycerol 26.78 g/L, yeast extract 10 g/L, buckwheat filtrate 20 g/L, soluble starch 3.03 g/L, MgSO4·7H2O 0.5 g/L, FeSO4·7H2O 0.01 g/L, KH2PO4, 0.25 g/L, NH4Cl 1 g/L, CaCO3 5 g/L, and pH 7.4. Fermentation was carried out in 250 mL flasks containing 50 mL of medium and the cells were cultivated at 28 °C, 220 rpm for 8 days
  3. cThe detailed component in medium (Du et al. 2017) was glucose 5 g/L, glycerol 40 g/L, sucrose 2.39 g/L, yeast extract 10 g/L, KH2PO4·3H2O 0.65 g/L, MgSO4·7H2O 0.5 g/L, FeSO4·7H2O 0.002 g/L, CaCO3 2 g/L, and pH 7.45. Fermentation was carried out in 250 mL flasks containing 60 mL of medium and the cells were cultivated at 28 °C, 220 rpm for 12 days