| Genotype/description | References/source |
---|---|---|
S. rimosus strains | ||
 M4018 | The genetically most characterized strain, the oxytetracycline-producing Pfizer strain | (Rhodes et al. 1984) |
 M4018 (pIB-KA) | Integrated a control plasmid pIB-KA in the genome of M4018 | This study |
 M4018 (pIB-KA-rim) | rimA1A2-overexpressed strain, derived from M4018 | This study |
 M4018 Δrim | rimA1-disrupted mutant, derived from M4018 | This study |
 M4018 Δrim (pIB-KA) | Integrated a control plasmid pIB-KA in the genome of M4018 Δrim | This study |
 M4018 Δrim (pIB-KA-rim) | RimA1A2-complemented strain, derived from M4018 Δrim | This study |
E. coli strains | ||
 DH5α | Used for routine subcloning | Novagen |
 ET12567 (pUZ8002) | Donor strain for conjugation between E. coli and Streptomyces | (MacNeil et al. 1992) |
Plasmids | ||
 pMD19-TS | T vector with ampicillin resistance gene | Takara |
 pET-28a | The donor of the kanamycin resistance fragment | Novagen |
 pKC1139 | An E. coli–Streptomyces shuttle vector containing the apramycin resistance gene aac(3)IV, the oriT of plasmid RP4, and a temperature-sensitive replicon | (Bierman et al. 1992) |
 pKC1139-rim | For disruption of rimA1 gene, containing part of rimA1 gene | This study |
 pIB139 | attP-int derived integration vector for the conjugal transfer of DNA from E. coli to Streptomyces, containing the apramycin resistance gene aac(3)IV and the ermE* promoter | (Wilkinson et al. 2002) |
 pIB-KA | Additional kanamycin resistance genes inserted into pIB139 | This study |
 pIB-KA-rim | pIB-KA derivative harboring the complete rimA1A2 gene under the control of PermE* | This study |