Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Study on a two-component signal transduction system RimA1A2 that negatively regulates oxytetracycline biosynthesis in Streptomyces rimosus M4018

 

Genotype/description

References/source

S. rimosus strains

 M4018

The genetically most characterized strain, the oxytetracycline-producing Pfizer strain

(Rhodes et al. 1984)

 M4018 (pIB-KA)

Integrated a control plasmid pIB-KA in the genome of M4018

This study

 M4018 (pIB-KA-rim)

rimA1A2-overexpressed strain, derived from M4018

This study

 M4018 Δrim

rimA1-disrupted mutant, derived from M4018

This study

 M4018 Δrim (pIB-KA)

Integrated a control plasmid pIB-KA in the genome of M4018 Δrim

This study

 M4018 Δrim (pIB-KA-rim)

RimA1A2-complemented strain, derived from M4018 Δrim

This study

E. coli strains

 DH5α

Used for routine subcloning

Novagen

 ET12567 (pUZ8002)

Donor strain for conjugation between E. coli and Streptomyces

(MacNeil et al. 1992)

Plasmids

 pMD19-TS

T vector with ampicillin resistance gene

Takara

 pET-28a

The donor of the kanamycin resistance fragment

Novagen

 pKC1139

An E. coli–Streptomyces shuttle vector containing the apramycin resistance gene aac(3)IV, the oriT of plasmid RP4, and a temperature-sensitive replicon

(Bierman et al. 1992)

 pKC1139-rim

For disruption of rimA1 gene, containing part of rimA1 gene

This study

 pIB139

attP-int derived integration vector for the conjugal transfer of DNA from E. coli to Streptomyces, containing the apramycin resistance gene aac(3)IV and the ermE* promoter

(Wilkinson et al. 2002)

 pIB-KA

Additional kanamycin resistance genes inserted into pIB139

This study

 pIB-KA-rim

pIB-KA derivative harboring the complete rimA1A2 gene under the control of PermE*

This study