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Table 1 Bacterial strains and plasmids used in this study

From: Study on a two-component signal transduction system RimA1A2 that negatively regulates oxytetracycline biosynthesis in Streptomyces rimosus M4018

  Genotype/description References/source
S. rimosus strains
 M4018 The genetically most characterized strain, the oxytetracycline-producing Pfizer strain (Rhodes et al. 1984)
 M4018 (pIB-KA) Integrated a control plasmid pIB-KA in the genome of M4018 This study
 M4018 (pIB-KA-rim) rimA1A2-overexpressed strain, derived from M4018 This study
 M4018 Δrim rimA1-disrupted mutant, derived from M4018 This study
 M4018 Δrim (pIB-KA) Integrated a control plasmid pIB-KA in the genome of M4018 Δrim This study
 M4018 Δrim (pIB-KA-rim) RimA1A2-complemented strain, derived from M4018 Δrim This study
E. coli strains
 DH5α Used for routine subcloning Novagen
 ET12567 (pUZ8002) Donor strain for conjugation between E. coli and Streptomyces (MacNeil et al. 1992)
Plasmids
 pMD19-TS T vector with ampicillin resistance gene Takara
 pET-28a The donor of the kanamycin resistance fragment Novagen
 pKC1139 An E. coliStreptomyces shuttle vector containing the apramycin resistance gene aac(3)IV, the oriT of plasmid RP4, and a temperature-sensitive replicon (Bierman et al. 1992)
 pKC1139-rim For disruption of rimA1 gene, containing part of rimA1 gene This study
 pIB139 attP-int derived integration vector for the conjugal transfer of DNA from E. coli to Streptomyces, containing the apramycin resistance gene aac(3)IV and the ermE* promoter (Wilkinson et al. 2002)
 pIB-KA Additional kanamycin resistance genes inserted into pIB139 This study
 pIB-KA-rim pIB-KA derivative harboring the complete rimA1A2 gene under the control of PermE* This study