Table 2 Primers used in this study
Primers | Sequence (5′–3′) |
|---|---|
PCR amplification | |
 DrimF | CCCAAGCTTACACCTCGACGATGTTGTTG |
 DrimR | TGCTCTAGAATACGCGGTCGATGTGGTG |
 aprF | GTGCAATACGAATGGCGAAAA |
 aprR | TCAGCCAATCGACTGGCGA |
 rim-single-P1 | AACCGGTAGTCCGCCGTACGGTG |
 rim-single-P2 | AGGTTGAGAAGCTGACCGATGAGCTC |
 pKANTTF | CTAGCTAGCCTCAGTGGAACGAAAACTCAC |
 pKANTTR | CTAGCTAGCACAATTTCAGGTGGCACTTTTC |
 rim-pIBF | CGGAATTCCTACGCCACCGGCAGGCGTA |
 rim-pIBR | GGAATTCCATATGCGCCTGTTGATCGTGGAG |
 riml | CTACGCCACCGGCAGGCGTAGTTC |
 rimR | ATGCGCCTGTTGATCGTGGAGGAC |
 attLf | GTTCACCCACAGCTGGAGGCC |
 attLr | GCTCGACTTCGCGCTGAAGGT |
 attRf | GCTATAATGACCCCGAAGCAG |
 attRr | TCGTCATGCCCCGCAGT |
RT-PCR | |
 co-hrdF | GTCCAGGTAGTCGCGCAGGAC |
 co-hrdR | TCTCGCTGCACACCCCGCTG |
 co-rimF | GTCGCGATCTGCGTGGCGAC |
 co-rimR | TGCTGCGCGTGGGAGAGCTG |
RT-qPCR | |
 oxyB-F | ACGTCAGCGCGTATCAGTCCTTC |
 oxyB-R | GCACAGCGAACCGTCCACAC |
 otrB-F | TTCGCCTTCCTCCAGATGGCGTG |
 otrB-R | AGCGGTACGTTGAGCAGGAACAC |
 otrC-F2 | TGCTGTATCTGGACGAGCCGAC |
 otrC-R2 | GTCGATGACCGTCAGCTCGTG |
 otcG-F | ATGTCATTCTGCTGGACGGGTC |
 otcG-R | TCAGAAATCCGCTCACGCGCAG |
 otcR-F | ATGAAGTCCTTGCGCAGCTGGTAG |
 otcR-R | CTCCGAAAGTGAAGCAGGTGCTC |
 hrdB-F | AGAGCGTCGCAGCGAAGAGTC |
 hrdB-R | CTTCTTGGCGACCGTCTTCTTCTC |