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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems

Fig. 3

Strategy and verification of gene replacement by CRISPR. a Schematic for replacement of agdF by glaA expression cassette mediated by CRISPR/Cas9 system. b Schematic for PCR verification of mutants. The expected length of fragments amplified from both up- and down-stream of agdF locus in genomes of mutants was about 1600 bp, while there is no product amplified from the genome template of WT strain CBS513.88. c The PCR results of mutants and WT strain. Five single colonies were separated from the primary transformant with the WT strain CBS513.88 as the negative control (U means results of upstream PCR and D means results of downstream PCR; 1–5 stands for the 5 mutant candidates and W stands for WT strain.) The results confirmed that expected gene replacement was arisen in 3 mutants (1, 3 and 4)

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