Fig. 6
From: Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems
Verification of genotype by PCR in the selected transformants after co-transformation of repair fragments with different lengths of homologous arms. The expected length for amplified product from agdF::glaA mutants was about 820 bp for both up- and down-stream PCR, while no band will be produced from the wide type strain CBS513.88. a PCR results using homologous arm of 500 bp. Results showed that all 3 tested candidates had the expected genotype. b PCR results using homologous arm of 100 bp. Results showed that all 5 tested candidates had the expected genotype