Table 1 Primers used in the study
From: Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems
Primer name | Sequence (5′–3′) |
|---|---|
Cas9-F | GCCTGAACTCACCGCGACGTGACTATAAGGACCACGACGG |
Cas9-R | TCAGTAACGTTAAGTGGATCCTTTTTCTTTTTTGCCTGGC |
HindIII-sgRNA-F | AACTCACCGCGACGTAAGCTTATGACCCAATACACCGCT |
HindIII-sgRNA-R | CAGTAACGTTAAGTGACTAGTGACCCAATGTCCCATTCG |
hyg-F | GTACTGTGTAAGCGCTCCGTCTCCATTGGCTCT |
hyg-R | GTACTGAGAGTGCAGCAAGGAGATGGCGCCCAA |
P1 | AACTCACCGCGACGTTTACATCTGATGAGTCCGTGAGGA |
P2 | CTCAACGGACGCTGTTACATGACGAGCTTACTCGTTTC |
P3 | ATGTAACAGCGTCCGTTGAGGTTTTAGAGCTAGAAATAGC |
P4 | CAGTAACGTTAAGTGTCGAGATGACCCAATGT |
PU1 | AATTCAACCTCAGCCCTA |
PU2 | TGGCTCTTAATGAGCTGG |
PD1 | CCTACAGGACACACATTC |
PD2 | CAAATTCCACAGTCTCGG |