Table 3 Specific activity of enzymatic fractions and purification efficiency by compared to the crude extract
Sample | Enzymatic activity | Specific activity in purified fractions (U/mg) | Specific activity in biocatalyst crude extract (U/mg) | Fold purification |
|---|---|---|---|---|
Fraction D | Protease | 7.70 | 0.582 | 13 |
Fraction B | β-Glucosidase | 14.3 | 0.057 | 251 |
Fraction A-65%-2a | Endo-xylanase | 42.8 | 0.596 | 72 |
Fraction A-65%-2b | Endo-xylanase | 69.2 | 0.596 | 116 |
Fraction A-100% | Polygalacturonase | 1882 | 19.7 | 96 |
Fraction A-65%-1 | β-Glucanase | 187 | 0.781 | 239 |
Fraction C | Cellulase | 39.4 | 0.628 | 63 |