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Table 2 Biological extraction methods for recovery of chitin

From: Seafood waste: a source for preparation of commercially employable chitin/chitosan materials

S. no Source Microorganism Type of conditions Outcomes References
1. Shrimp waste Natural probiotic 72-h incubation, 5% inoculum level, 15% glucose concentration Rate of deproteination: 89%, rate of demineralization: 69% Prameela et al. (2010)
2. Shrimp waste Pseudomonas aeruginosa A2 Temperature 60 °C and pH 8.0 DM 3 h hydrolysis at 40 °C Rate of deproteination: 56% Ghorbel-Bellaaj et al. (2011)
3. Shrimp waste Lactobacillus plantarum PTCC 1058- Medium-shrimp shell powder (5% w/v), peptone, yeast extract, meat extract, K2HPO4, Na-acetate, (NH4)2-citrate, Rate of demineralization: 82% Khorrami et al. (2011)
4. Shrimp waste Lactobacillus plantarum 1058 5% of seed culture, fermentation at 30 °C, 180 rpm, 6 days Rate of deproteination: 45%, rate of demineralization: 55% Khorrami et al. (2012)
5. Crab waste Lactobacillus sp. B2 1 (vv−1%) of Lactobacillus sp. B2 30 °C, 200 rpm, 120 h Rate of deproteination: 56%, rate of demineralization: 88%, chitin purity 34% Flores-Albino et al. (2012)
6. Shrimp waste Bacillus licheniformis 5% (w/v) glucose, initial pH 7, 5 days at 37 °C, 200 rpm. Rate of demineralization: 55% Ghorbel-Bellaaj et al. (2012a, b)
7. Shrimp waste B. cereus SV1 5% (w/v) glucose, initial pH 7, 5 days at 37 °C, 200 rpm. 95% rate of deproteination Ghorbel-Bellaaj et al. (2012a, b)
8. Crab, shrimp, prawn, krill and lobster Bacillus licheniformis NRS-1264, Bacillus subtilis B-59994 pH 7, 40 ± 1 °C, 150 rpm Rate of demineralization: 62.5% Pachapur et al. (2016)
9. Shrimp waste Pseudomonas aeruginosa 20% glucose, 20% inoculation and 6 days fermentation 82% demineralization, 92% deproteinization, chitin yield: 47% Sedaghat et al. (2017)