Skip to main content

Table 2 Biological extraction methods for recovery of chitin

From: Seafood waste: a source for preparation of commercially employable chitin/chitosan materials

S. no

Source

Microorganism

Type of conditions

Outcomes

References

1.

Shrimp waste

Natural probiotic

72-h incubation, 5% inoculum level, 15% glucose concentration

Rate of deproteination: 89%, rate of demineralization: 69%

Prameela et al. (2010)

2.

Shrimp waste

Pseudomonas aeruginosa A2

Temperature 60 °C and pH 8.0 DM 3 h hydrolysis at 40 °C

Rate of deproteination: 56%

Ghorbel-Bellaaj et al. (2011)

3.

Shrimp waste

Lactobacillus plantarum PTCC 1058-

Medium-shrimp shell powder (5% w/v), peptone, yeast extract, meat extract, K2HPO4, Na-acetate, (NH4)2-citrate,

Rate of demineralization: 82%

Khorrami et al. (2011)

4.

Shrimp waste

Lactobacillus plantarum 1058

5% of seed culture, fermentation at 30 °C, 180 rpm, 6 days

Rate of deproteination: 45%, rate of demineralization: 55%

Khorrami et al. (2012)

5.

Crab waste

Lactobacillus sp. B2

1 (vv−1%) of Lactobacillus sp. B2 30 °C, 200 rpm, 120 h

Rate of deproteination: 56%, rate of demineralization: 88%, chitin purity 34%

Flores-Albino et al. (2012)

6.

Shrimp waste

Bacillus licheniformis

5% (w/v) glucose, initial pH 7, 5 days at 37 °C, 200 rpm.

Rate of demineralization: 55%

Ghorbel-Bellaaj et al. (2012a, b)

7.

Shrimp waste

B. cereus SV1

5% (w/v) glucose, initial pH 7, 5 days at 37 °C, 200 rpm.

95% rate of deproteination

Ghorbel-Bellaaj et al. (2012a, b)

8.

Crab, shrimp, prawn, krill and lobster

Bacillus licheniformis NRS-1264, Bacillus subtilis B-59994

pH 7, 40 ± 1 °C, 150 rpm

Rate of demineralization: 62.5%

Pachapur et al. (2016)

9.

Shrimp waste

Pseudomonas aeruginosa

20% glucose, 20% inoculation and 6 days fermentation

82% demineralization, 92% deproteinization, chitin yield: 47%

Sedaghat et al. (2017)