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Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions

Fig. 6

Cells were cultured with 5 uM SB216763, 1 uM PD0325901, 2 uM SU5402 or their combination. A Alkaline phosphatase staining. Scale bar: 100 μm. B DAPI staining for nuclei. C Immunofluorescence staining for OCT4. Scale bar: 100 μm. D Semi-quantitative RT-PCR analysis of Sox2, Oct4 and Nanog mRNA expression

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