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Table 7 The results of analysis of orthogonal experimental design

From: Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation

Calculation

Glycerol concentration

(g/L) [A]

pH

[B]

C/N ratio (g/g)

[C]

K1

2.54

2.97

2.64

K2

2.61

2.41

2.89

K3

3.06

2.83

2.68

k 1

0.85

0.99

0.88

k 2

0.87

0.80

0.96

k 3

1.02

0.94

0.89

R

0.17

0.19

0.08

Primary and secondary order

B > A > C

Optimal levels

A2

B1

C2

Optimal combination

10 g/L, pH5.0, C/N 5/3