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Bioresources and Bioprocessing

Table 1 Results of site-directed mutagenesis of A85 and I86

From: Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones

Enzymes

c (%)a

ee (%)b

WT

–

–

A85G

6

96(S)

I86A

13

80(S)

A85G/I86A

76

> 99(S)

  1. aConversion rate (c %) was determined by high-performance liquid chromatography (HPLC) analysis as shown in Additional file 1
  2. bThe ee values were determined by chiral HPLC analysis; the absolute configuration was determined by comparison of the elution order with literature data (see Additional file 1)
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