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Bioresources and Bioprocessing

Table 4 Kinetic parameters of TbSADH mutants towards substrate 1

From: Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones

Enzymes

1

NADPH

Km (mM)

Kcat (s−1)

kcat/Km (s−1 mM−1)

Km (mM)

kcat (s−1)

kcat/Km (s−1 mM−1)

A85G/I86A

0.095 ± 0.01

8.50 ± 0.21

89.47

0.042 ± 0.01

24.51 ± 1.51

583.57

A85G/I86A/Q101A

0.079 ± 0.02

28.92 ± 0.22

366.08

0.06 ± 0.01

32.48 ± 0.95

541.33

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