Entry | Substrate code | Enzymes | |||||||
---|---|---|---|---|---|---|---|---|---|
WT | A85G/I86A | A85G/I86A/C295A | A85G/I86A/Q101A | ||||||
c (%)a | ee (%)b | c (%)a | ee (%)b | c (%) | ee (%)b | c (%)a | ee (%)b | ||
1 | 3a | 98 | 97(S) | 49 | 48(R) | 49 | 56(R) | 1 | – |
2 | 3b | 6 | 65(S) | – | – | 49 | 96(S) | 2 | – |
3 | 3c | 5 | 40(S) | – | – | 35 | 98(S) | 3 | – |
4 | 3d | < 1 | – | – | – | 11 | > 99(R) | – | – |
5 | 3e | 2 | – | – | – | 3 | – | < 1 | – |
6 | 3f | 16 | 84(S) | – | – | 6 | 75(R) | – | – |
7 | 3g | 41 | > 99(R) | 42 | > 99(R) | 34 | 98(R) | 1 | – |
8 | 3h | 8 | 78(R) | 38 | 98(S) | 93 | 93(R) | > 99 | > 99(R) |
9 | 3i | > 99 | 74(R) | > 99 | 52(R) | 95 | 54(R) | 98 | 41(R) |
10 | 3j | > 99 | 26(S) | > 99 | > 99(R) | 98 | 97(R) | 98 | 95(R) |
11 | 3k | 13 | 36(S) | 15 | 44(R) | 12 | 41(R) | 14 | 43(R) |
12 | 3l | 85 | 75(R) | 94 | > 99(R) | 83 | > 99(R) | 44 | > 99(R) |
13 | 3m | 98 | 36(S) | 98 | 11(S) | 78 | 77(S) | < 1 | – |
14 | 3n | 68 | 66(R) | 98 | > 99(S) | 99 | > 99(S) | > 99 | > 99(R) |