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Bioresources and Bioprocessing

Table 3 Kinetic parameters for ABTS oxidation of DyP4 and its variants

From: Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY)

DyP4 and variants

Km (mM)

Vmax (mM−1 s−1)

kcat (s−1)

kcat/Km (mM−1 s−1)

WT

0.895 ± 0.065

6.779 ± 0.142 × 10−4

3.86 ± 0.08

4.31 ± 0.41

3F6

0.427 ± 0.055

6.877 ± 0.202 × 10−4

5.02 ± 0.15

11.78 ± 1.86

4D4

0.390 ± 0.059

6.966 ± 0.233 × 10−4

4.52 ± 0.15

11.60 ± 2.14

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