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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Fig. 2

SDS-PAGE analysis of recombinant enzyme expression in E. coli BL21 (DE3) and purification of AfPase. M, protein marker; T, total cell lysate of recombinant E. coli cells; S, the supernatant of cell lysate; 70H, purified AfPase after heat treatment of cell supernatant at 70 °C for 20 min; 80H, purified AfPase after heat treatment of cell supernatant at 80 °C for 20 min; 90H, purified AfPase after heat treatment of cell supernatant at 90 °C for 20 min; Ni–NTA, purified AfPase by Ni–NTA resin

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