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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Fig. 4

Effects of pH on the specific activity of AfPase. The reactions were performed in 100 mM glycine–HCl buffer (pH 4.0–5.0), acetic acid–acetate sodium buffer (pH 5.0–6.0), Bis–Tris buffer (pH 6.0–7.0), HEPES buffer (pH 7.0–8.0) containing 1 mM p-nitrophenyl phosphate, 5 mM MgCl2, and appropriate concentration of phosphatase at 85 °C

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