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Table 1 Purification of AfPase from 300 mL of the E. coli culture

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Fraction Vol. (mL) Pro. (mg/mL) Total pro. (mg) Sp. act. (U/mg)a Total act. (U) Yield (%)
Supernatant 10 18.63 186.3 13.2 2459 100
70Hb 8.3 7.14 59.3 26.7 1583 64
80H 8.3 7.11 58.1 25.9 1505 61
90H 8.3 6.72 55.8 26.9 1501 61
Ni–NTA 16 1.42 22.7 19.1 434 18
  1. aThe activity assay was performed in 100 mM HEPES buffer (pH 7.0) containing 1 mM p-nitrophenyl phosphate, 5 mM Mg2+ at 85 °C
  2. b70H indicates that the enzymes were purified by heat treatment at 70 °C for 20 min, the same situations for 80H and 90H