Bioresources and Bioprocessing

Table 1 Purification of AfPase from 300 mL of the E. coli culture

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Fraction	Vol. (mL)	Pro. (mg/mL)	Total pro. (mg)	Sp. act. (U/mg)^a	Total act. (U)	Yield (%)
Supernatant	10	18.63	186.3	13.2	2459	100
70H^b	8.3	7.14	59.3	26.7	1583	64
80H	8.3	7.11	58.1	25.9	1505	61
90H	8.3	6.72	55.8	26.9	1501	61
Ni–NTA	16	1.42	22.7	19.1	434	18

^aThe activity assay was performed in 100 mM HEPES buffer (pH 7.0) containing 1 mM p-nitrophenyl phosphate, 5 mM Mg²⁺ at 85 °C
^b70H indicates that the enzymes were purified by heat treatment at 70 °C for 20 min, the same situations for 80H and 90H

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