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Bioresources and Bioprocessing

Table 2 Promiscuity of AfPase

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Substrate

Rate (μmol/min/mg)

p-Nitrophenyl phosphate

3.36 ± 0.21

Glucose 1-phosphate

0.0018

Glucose 6-phosphate

0.002

Glucosamine 6-phosphate

ND

Fructose 6-phosphate

0.0057

Tagatose 6-phosphate

ND

Psicose 6-phosphate

ND

Mannose 6-phosphate

ND

Fructose 1,6-diphosphate

0.0162

Glyceraldehyde 3-phosphate

0.38 ± 0.02

Dihydroxyacetone phosphate

1.03 ± 0.09

Deoxyribose 5-phosphate

0.06 ± 0.005

  1. Values were measured at 60 °C in 100 mM HEPES buffer (pH 7.0), containing 10 mM substrate and 5 mM MgCl2
  2. ND not detected (less than 0.001)
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