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Table 2 Promiscuity of AfPase

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Substrate Rate (μmol/min/mg)
p-Nitrophenyl phosphate 3.36 ± 0.21
Glucose 1-phosphate 0.0018
Glucose 6-phosphate 0.002
Glucosamine 6-phosphate ND
Fructose 6-phosphate 0.0057
Tagatose 6-phosphate ND
Psicose 6-phosphate ND
Mannose 6-phosphate ND
Fructose 1,6-diphosphate 0.0162
Glyceraldehyde 3-phosphate 0.38 ± 0.02
Dihydroxyacetone phosphate 1.03 ± 0.09
Deoxyribose 5-phosphate 0.06 ± 0.005
  1. Values were measured at 60 °C in 100 mM HEPES buffer (pH 7.0), containing 10 mM substrate and 5 mM MgCl2
  2. ND not detected (less than 0.001)