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Table 3 Kinetic parameters of AfPase against DHAP in 100 mM HEPES (pH 7.0) containing 5 mM MgCl2 at different temperatures

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Temperature kcat (/s) Km (mM) kcat/Km (/mM/s)
60 °C 0.64 4.72 0.136
37 °C 0.16 10.47 0.015