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Table 3 Kinetic parameters of AfPase against DHAP in 100 mM HEPES (pH 7.0) containing 5 mM MgCl2 at different temperatures

From: Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem

Temperature

kcat (/s)

Km (mM)

kcat/Km (/mM/s)

60 °C

0.64

4.72

0.136

37 °C

0.16

10.47

0.015