Table 2 Error-prone PCR system
From: Improving the specific activity and pH stability of xylanase XynHBN188A by directed evolution
Component | Reaction system ONE (μl) | Reaction system TWO (μl) |
|---|---|---|
template plasmid pET28a-xynHBN188A (50 ng/μl) | 2.00 | 2.00 |
18N188AF (20 μM) | 2.50 | 2.50 |
18N188AR (20 μM) | 2.50 | 2.20 |
dATP (10 mM) | 2.00 | 2.00 |
dCTP (10 mM) | 2.00 | 2.00 |
dGTP (10 mM) | 1.80 | 3.40 |
dTTP (10 mM) | 1.26 | 2.36 |
MgCl2 (50 mM) | 4.08 | 6.64 |
BSA (0.1 μg/ul) | 5.00 | 5.00 |
Ni Taq DNA Polymerase | 1.00 | 1.00 |
MnCl2 (5 mM) | 10.00 | 10.00 |
10 × PCR Buffer (without Mg2+) | 10.00 | 10.00 |
ddH2O | 55.86 | 50.40 |