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Table 2 Error-prone PCR system

From: Improving the specific activity and pH stability of xylanase XynHBN188A by directed evolution

ComponentReaction system ONE (μl)Reaction system TWO (μl)
template plasmid pET28a-xynHBN188A (50 ng/μl)2.002.00
18N188AF (20 μM)2.502.50
18N188AR (20 μM)2.502.20
dATP (10 mM)2.002.00
dCTP (10 mM)2.002.00
dGTP (10 mM)1.803.40
dTTP (10 mM)1.262.36
MgCl2 (50 mM)4.086.64
BSA (0.1 μg/ul)5.005.00
Ni Taq DNA Polymerase1.001.00
MnCl2 (5 mM)10.0010.00
10 × PCR Buffer (without Mg2+)10.0010.00
ddH2O55.8650.40