Enhanced lincomycin A production by calcium gluconate feeding in fermentation of Streptomyces lincolnensis

Bioresources and Bioprocessing

Table 1 The production of lincomycin A with various feeding modes of gluconate

Feeding mode	Concentration of gluconate (g/L)	Addition time or period (h)	Speed of continuous addition (g/L/h)	Starting titer of lincomycin A (mg/L)	Lincomycin A (mg/L)	DCW (g/L)
Control	–	–	–	562 ± 32^b	6480 ± 219	45 ± 2.1
Mode 1	2 + 2^a	0 + 127^a	–	590 ± 41	7646 ± 368	49 ± 1.9
Mode 2	2 + 2	0 + 147	–	592 ± 42	7776 ± 312	49 ± 2.3
Mode 3	1.8 + 2.2	0 + 147	–	646 ± 51	7832 ± 297	48 ± 2.4
Mode 4	1.2 + 2.8	0 + 147	–	741 ± 48	7981 ± 323	47 ± 2.6
Mode 5	0.6 + 3.4	0 + 147	–	921 ± 78	8012 ± 345	43 ± 2.1
Mode 6	0.6 + 3.4	0 + 111–168	0.0512	910 ± 58	7220 ± 228	43 ± 2.4
Mode 7	0.6 + 3.4	0 + 111–163	0.0575	931 ± 76	7456 ± 321	44 ± 2.3
Mode 8	0.6 + 3.4	0 + 111–158	0.0638	916 ± 56	9160 ± 316	45 ± 3.1
Mode 9	0.6 + 3.4	0 + 111–153	0.0701	932 ± 73	8338 ± 225	48 ± 3.5

^a“2 + 2” and “0 + 127” mean that there was 2 g/L calcium gluconate added in the bioreactor at 0 h, and 2 g/L calcium gluconate added in the bioreactor at 127 h during the fermentation process
^bStarting titer of lincomycin A was measured from the sample taken at 54 h during the fermentation process