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Table 1 Kinetic parameters of UDH from wt and mutants toward substrate glucuronic acid

From: Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering

Mutants kcat (102s−1) Km (mM) kcat/Km (102 s−1 mM−1) Specific activity (U mg−1) Relative activity (100%)
WT 1.61 ± 0.3 0.20 ± 0.06 8.0 85 ± 2.1 100.0 ± 3.2
A39P 1.92 ± 0.5 0.26 ± 0.03 7.3 88 ± 3.1 103.5 ± 2.6
H99Y 1.79 ± 0.4 0.23 ± 0.05 7.7 93 ± 4.1 109.6 ± 3.4
H234K 1.95 ± 0.2 0.24 ± 0.03 8.1 110 ± 3.1 129.4 ± 2.9
A39P/H99Y 2.39 ± 0.1 0.20 ± 0.04 7.2 124 ± 2.9 146.8 ± 3.2
A39P/H234K 2.95 ± 0.4 0.18 ± 0.06 8.4 140 ± 3.6 165.7 ± 3.5
H99Y/H234K 3.18 ± 0.5 0.17 ± 0.05 8.6 149 ± 1.9 175.4 ± 2.9
A39P/H99Y/H234K 3.8 ± 0.7 0.15 ± 0.03 9.1 210 ± 3.0 247.6 ± 3.5