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Table 2 Thermostability and activity parameters of wild-type and mutants of AtLBA 4404 UDH

From: Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering

Enzyme

Relative activity (30 °C)

t1/2 (min) (59 °C)

Improvement of t1/2 (59 °C)

\( \Delta {\text{T}}_{ 5 0}^{ 1 0} \)

WT

85 ± 2.1

0.30 ± 0.02

1

0

A39P

88 ± 3.1

0.60 ± 0.1

1.8

0

H99Y

93 ± 4.1

1.30 ± 0.5

4.3

2

H234K

110 ± 3.1

5.10 ± 1.1

17

2.5

A39P/H99Y

124 ± 2.9

1.10 ± 0.5

3.5

3

A39P/H234K

140 ± 3.6

6.30 ± 1.9

21

3.5

H99Y/H234K

149 ± 1.9

16.00 ± 2.5

53

4

A39P/H99Y/H234K

210 ± 3.0

120 ± 3.5

400

5