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Table 2 Thermostability and activity parameters of wild-type and mutants of AtLBA 4404 UDH

From: Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering

Enzyme Relative activity (30 °C) t1/2 (min) (59 °C) Improvement of t1/2 (59 °C) \( \Delta {\text{T}}_{ 5 0}^{ 1 0} \)
WT 85 ± 2.1 0.30 ± 0.02 1 0
A39P 88 ± 3.1 0.60 ± 0.1 1.8 0
H99Y 93 ± 4.1 1.30 ± 0.5 4.3 2
H234K 110 ± 3.1 5.10 ± 1.1 17 2.5
A39P/H99Y 124 ± 2.9 1.10 ± 0.5 3.5 3
A39P/H234K 140 ± 3.6 6.30 ± 1.9 21 3.5
H99Y/H234K 149 ± 1.9 16.00 ± 2.5 53 4
A39P/H99Y/H234K 210 ± 3.0 120 ± 3.5 400 5