Enzyme | Relative activity (30 °C) | t1/2 (min) (59 °C) | Improvement of t1/2 (59 °C) | \( \Delta {\text{T}}_{ 5 0}^{ 1 0} \) |
---|---|---|---|---|
WT | 85 ± 2.1 | 0.30 ± 0.02 | 1 | 0 |
A39P | 88 ± 3.1 | 0.60 ± 0.1 | 1.8 | 0 |
H99Y | 93 ± 4.1 | 1.30 ± 0.5 | 4.3 | 2 |
H234K | 110 ± 3.1 | 5.10 ± 1.1 | 17 | 2.5 |
A39P/H99Y | 124 ± 2.9 | 1.10 ± 0.5 | 3.5 | 3 |
A39P/H234K | 140 ± 3.6 | 6.30 ± 1.9 | 21 | 3.5 |
H99Y/H234K | 149 ± 1.9 | 16.00 ± 2.5 | 53 | 4 |
A39P/H99Y/H234K | 210 ± 3.0 | 120 ± 3.5 | 400 | 5 |