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Table 1 Typical procedures of sample preparation for Streptomyces

From: Optimized sampling protocol for mass spectrometry-based metabolomics in Streptomyces

Strains

Samplinga

Separationb

Washing

Quenchingd

Extractione

Reference strains

Streptomyces albus (Lu et al. 2016)

9 mL sample

Centrifuge

Noc

Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v) to a final volume of 1 mL − 20 °C

Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v); 1 mL; − 20 °C

Escherichia coli

Streptomyces avermitilis (Guo et al. 2015)

1 sampleg

Centrifuge

6 mL 0.6% (w/v) NaCl at 4 °C

4 g 40% methanol at − 40 °C

1 mL; chloroform: ethanol: water = 2:2:1 (v/v/v), thawed and frozen

Yeast

Streptomyces coelicolor (Wentzel et al. 2012b)

5 mL sample

Filtration

5 mL 2.63% (w/v) NaCl

25 mL 60% methanol at − 23 °C

25 mL 60% methanol at − 23 °C, thawed and frozen

Nullf

Streptomyces hygroscopicus (Wang et al. 2015)

10 mL sample

Centrifuge

10 mL 0.9% (w/v) NaCl at 4 °C

40 mL 60% methanol at − 40 °C

Grinded using liquid nitrogen; 1.0 mL cooled methanol (50% (v/v), − 40 °C); thawed and frozen

Streptomyces coelicolor

Streptomyces lividans (Muhamadali et al. 2015)

15 mL sample

Centrifuge

Noc

30 mL 60% methanol at − 45 °C

0.5 mL 100% methanol at − 45 °C, thawed and frozen

Escherichia coli

Streptomyces lydicus (Cheng et al. 2013)

  

Deionized water

60% methanol at − 40 °C

Grinded using liquid nitrogen; cooled methanol (50% (v/v), − 40 °C)

Saccharomyces cerevisiae

Streptomyces tsukubaensis (Xia et al. 2013)

10 mL sample

Filtration

10 mL 0.9% (w/v) NaCl

25 mL 60% methanol at − 40 °C

2.5 mL 50% (v/v) methanol at − 30 °C, thawed and frozen

Streptomyces coelicolor

Streptomyces lividans (Kassama et al. 2010)

5 mL sample

Centrifuge

Noc

25 mL 60% methanol containing 10 mM HEPES at − 40 °C

1 mL 100% methanol at − 40 °C, thawed and frozen

Nullf

  1. aSample volume for each sampling; b separation methods for separating pellet and broth; c no washing steps; d the ingredient of quenching solution and temperature; e the ingredient and operation of extraction and temperature; f developing methods for their own strains; g fourfold volume quenching solution was added to the sample