From: Optimized sampling protocol for mass spectrometry-based metabolomics in Streptomyces
Strains | Samplinga | Separationb | Washing | Quenchingd | Extractione | Reference strains |
---|---|---|---|---|---|---|
Streptomyces albus (Lu et al. 2016) | 9 mL sample | Centrifuge | Noc | Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v) to a final volume of 1 mL − 20 °C | Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v); 1 mL; − 20 °C | Escherichia coli |
Streptomyces avermitilis (Guo et al. 2015) | 1 sampleg | Centrifuge | 6 mL 0.6% (w/v) NaCl at 4 °C | 4 g 40% methanol at − 40 °C | 1 mL; chloroform: ethanol: water = 2:2:1 (v/v/v), thawed and frozen | Yeast |
Streptomyces coelicolor (Wentzel et al. 2012b) | 5 mL sample | Filtration | 5 mL 2.63% (w/v) NaCl | 25 mL 60% methanol at − 23 °C | 25 mL 60% methanol at − 23 °C, thawed and frozen | Nullf |
Streptomyces hygroscopicus (Wang et al. 2015) | 10 mL sample | Centrifuge | 10 mL 0.9% (w/v) NaCl at 4 °C | 40 mL 60% methanol at − 40 °C | Grinded using liquid nitrogen; 1.0 mL cooled methanol (50% (v/v), − 40 °C); thawed and frozen | Streptomyces coelicolor |
Streptomyces lividans (Muhamadali et al. 2015) | 15 mL sample | Centrifuge | Noc | 30 mL 60% methanol at − 45 °C | 0.5 mL 100% methanol at − 45 °C, thawed and frozen | Escherichia coli |
Streptomyces lydicus (Cheng et al. 2013) |  |  | Deionized water | 60% methanol at − 40 °C | Grinded using liquid nitrogen; cooled methanol (50% (v/v), − 40 °C) | Saccharomyces cerevisiae |
Streptomyces tsukubaensis (Xia et al. 2013) | 10 mL sample | Filtration | 10 mL 0.9% (w/v) NaCl | 25 mL 60% methanol at − 40 °C | 2.5 mL 50% (v/v) methanol at − 30 °C, thawed and frozen | Streptomyces coelicolor |
Streptomyces lividans (Kassama et al. 2010) | 5 mL sample | Centrifuge | Noc | 25 mL 60% methanol containing 10 mM HEPES at − 40 °C | 1 mL 100% methanol at − 40 °C, thawed and frozen | Nullf |