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Table 1 Typical procedures of sample preparation for Streptomyces

From: Optimized sampling protocol for mass spectrometry-based metabolomics in Streptomyces

Strains Samplinga Separationb Washing Quenchingd Extractione Reference strains
Streptomyces albus (Lu et al. 2016) 9 mL sample Centrifuge Noc Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v) to a final volume of 1 mL − 20 °C Acetonitrile/methanol/0.1% glacial acetate (45:45:10, v/v); 1 mL; − 20 °C Escherichia coli
Streptomyces avermitilis (Guo et al. 2015) 1 sampleg Centrifuge 6 mL 0.6% (w/v) NaCl at 4 °C g 40% methanol at − 40 °C 1 mL; chloroform: ethanol: water = 2:2:1 (v/v/v), thawed and frozen Yeast
Streptomyces coelicolor (Wentzel et al. 2012b) 5 mL sample Filtration 5 mL 2.63% (w/v) NaCl 25 mL 60% methanol at − 23 °C 25 mL 60% methanol at − 23 °C, thawed and frozen Nullf
Streptomyces hygroscopicus (Wang et al. 2015) 10 mL sample Centrifuge 10 mL 0.9% (w/v) NaCl at 4 °C 40 mL 60% methanol at − 40 °C Grinded using liquid nitrogen; 1.0 mL cooled methanol (50% (v/v), − 40 °C); thawed and frozen Streptomyces coelicolor
Streptomyces lividans (Muhamadali et al. 2015) 15 mL sample Centrifuge Noc 30 mL 60% methanol at − 45 °C 0.5 mL 100% methanol at − 45 °C, thawed and frozen Escherichia coli
Streptomyces lydicus (Cheng et al. 2013)    Deionized water 60% methanol at − 40 °C Grinded using liquid nitrogen; cooled methanol (50% (v/v), − 40 °C) Saccharomyces cerevisiae
Streptomyces tsukubaensis (Xia et al. 2013) 10 mL sample Filtration 10 mL 0.9% (w/v) NaCl 25 mL 60% methanol at − 40 °C 2.5 mL 50% (v/v) methanol at − 30 °C, thawed and frozen Streptomyces coelicolor
Streptomyces lividans (Kassama et al. 2010) 5 mL sample Centrifuge Noc 25 mL 60% methanol containing 10 mM HEPES at − 40 °C 1 mL 100% methanol at − 40 °C, thawed and frozen Nullf
  1. aSample volume for each sampling; b separation methods for separating pellet and broth; c no washing steps; d the ingredient of quenching solution and temperature; e the ingredient and operation of extraction and temperature; f developing methods for their own strains; g fourfold volume quenching solution was added to the sample