Fig. 6
From: Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment
Gene transcription study was performed using RT2 Profiler PCR array. A total of 84 genes were analyzed of which 12 were highly transcribed under the growth conditions, where the target molecule was oxidized. Transcription ranged from 2.02 to 7.04-fold over the oxidative gene control, bioreactor 8, which demonstrated no oxidation of the rFab molecule. Under similar growth conditions two oxidative protective genes glutathione synthetase and thyroid peroxidase were down regulated; transcription levels 0.426 and 0.46-fold less than control