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Fig. 9 | Bioresources and Bioprocessing

Fig. 9

From: Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment

Fig. 9

Schematic diagram illustrating a proposed pathway for rFab oxidation for cells under oxidative stress. The 12 genes identified in the red boxes were found to be highly transcribed in this study; AOX1, Aldehyde Oxidase 1; NOXO1, NADPH Oxidase Organizer 1; EPX, Eosinophil Peroxidase; NOX4, NADPH Oxidase 4; DUOX2, Dual Oxidase 2; UPC3, Uncoupling Protein 3; SOD3, Superoxide Dismutase 3; LOP, Lactoperoxidase; MPO, Myeloperoxidase; GSTP1, Glutathione S-Transferase; ALB, Serum Albumin Like; and NCOA7, Nuclear Receptor Coactivator 7. NOXO1, NOX4, DUOX2, AOX1, SOD3, MPO, LPO and EPX genes are all involved with the formation of ROSs (highlighted in the purple boxes), while UPC3 is responsible for pumping ROSs out of the mitochondria. GSTP1, ALB and NCOA7 are protective; their main function is to protect thiol containing proteins and lipids from oxidation by acting as a reducing agent and are not involved in reducing the level of H2O2 found within the cells

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